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Laboratory for Plant Cell Technology, Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University.

‹ÆÑiûM“c’B˜N y‹³ŽöjACHIEVEMENT

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2018”N
32. Fukuoka, N., Miyata, M., Hamada, T., Takeshita, E. 2019. Occurrence of internal browning in tuberous roots of sweetpotato and its related starch biosynthesis. Plant Physiol. Biochem. 135: 233-241. ¨PubMed

31. Fukuoka, N., Miyata, M., Hamada, T., Takeshita, E. 2018. Histochemical observations and gene expression changes related to internal browning in tuberous roots of sweet potato (Ipomea batatas). Plant Sci. 274: 476-484. ¨PubMed

2017”N
30.@Kitahara, K., Nakamura, Y., Otani, M., Hamada, T., Nakayachi, O., Takahata, Y. 2017. Carbohydrate components in sweetpotato storage roots: their diversities and genetic improvement. Breeding Sci. 67: 62-72.

29. Masuda, H., Shimochi, E., Hamada, T., Senoura, T., Kobayashi, T., Aung, M. S., Ishimaru, Y., Ogo, Y., Nakanishi, H., Nishizawa, N. K. 2017. A new transgenic rice line exhibiting enhanced ferric iron reduction and phytosiderophore production confers tolerance to low iron availability in calcareous soil. PLoS One. 12: e0173441. ¨PubMed

2015”N
28. Uwagaki, Y., Matsuda, E., Komaki, M., Murahama, M., Otani, M., Nishizawa, N., Hamada, T. 2015. Agrobacterium-mediated transformation and regeneration of Freesia ~ hybrida. Plant Biotech. 32: 165|168.

2014”N
27. Fukuoka, N., Suzuki, T. Minamide, K., Hamada, T. Effect of shading on anthocyanin and non-flavonoid polyphenol biosynthesis of Gynura bicolor leaves inmidsummer. Hort Science. 49: 1148|1153.

2012”N
26. Ishisakia, K., Araia, S., Hamada, T., Honda, Y. Biochemical characterization of a recombinant plant class III chitinase from the pitcher of the carnivorous plant Nepenthes alata. Carbohydrate Res. 361: 170|174. ¨PubMed

25. Hatano, N., Hamada, T. Proteomic analysis of secreted protein induced by a component of prey in pitcher fluid of the carnivorous plant Nepenthes alata. J. Proteomics. 75: 4844|4852. ¨PubMed

24. —LŽè—FŽkC“ìoŒ\—SC¬–q³ŽqCà_“c’B˜NC’|‘º”ü•ÛC‘åŽRŠÎŽ¢. u”\“o‘å”[Œ¾v‚ðŽ¯•Ê‚·‚éSSRƒ}[ƒJ[ƒZƒbƒg‚ÌŠJ”­D–k—¤ì•¨Šw‰ï•ñ. 47: 109|112D

23. Takemura, M., Hamada, T., Kida, H., Ohyama. K. Cold-induced accumulation of ω-3 polyunsaturated fatty acid in a liverwort, Marchantia polymorpha L. Biosci. Biotech. Biochem. 76: 785|790. ¨PubMed

22. Ishisaki, K., Honda, Y., Taniguchi, H., Hatano, N., Hamada, T. Heterogonous expression and characterization of a plant class IV chitinase from the pitcher of the carnivorous plant Nepenthes alata. Glycobiology. 22: 345|351. ¨PubMed

2011”N
21. Takemura, M., Okimura, Y., Kida, H., Hamada, T., Ohyama, K. Blue light enhances the accumulation of eicosapentaenoic acid in a liverwort, Marchantia polymorpha L. Plant Biotechnol. 28: 489|492.

2008”N
20. Hatano, N., Hamada, T. Proteome analysis of pitcher fluid of the carnivorous plant Nepenthes alata. J. Proteome Res. 7: 809|816. ¨PubMed

2007”N
19. Otani, M., Hamada, T., Katayama, K., Kitahara, K., Kim, SH.,Takahata, Y., Suganuma, T. Shimada, T. Inhibition of the gene expression for granule-bound starch synthase I by RNA interference in sweet potato plants. Plant Cell Rep. 26: 1801|1807. ¨PubMed

18. Kitahara, K., Hamasuna, K., Nozuma K., Otani, M., Hamada, T., Shimada, T., Fujita, K., Suganuma, T. Physicochemical properties of amylose-free and high-amylose starches from transgenic sweetpotatoes modified by RNA interference. Carbohydrate Polymers. 69: 233|240.

2006”N
17. Hamada, T., Kim, SH., Shimada, T. Starch-branching enzyme I gene (IbSBEI) from sweet potato (Ipomoea batatas); molecular cloning and expression analysis. Biotechnol. Lett. 28: 1255|1261. ¨PubMed

16. Hamada, T., Iba, K., Shimada, T. Reduction of trienoic fatty acid content by expression of a double-stranded RNA of a plastid ω-3 fatty acid desaturase gene in transgenic tobacco. Biotechnol. Lett. 28: 779|785. ¨PubMed

15. Kim, SH., Hamada, T., Kim, JS., Hong, JS. A novel J8 domain gene, IbJ8, in Ipomoea batatas (L). Lam. J. Plant Biol. 49: 212|217.

14. Shimada, T., Otani, M., Hamada, T., Kim, SH. Increase of amylose content of sweetpotato starch by RNA interference of the starch branching enzyme II gene (IbSBEII). Plant Biotechnol. 23: 85|90.

2005”N
13. Kim, SH., Hamada, T., Otani, M., Shimada, T. Rapid and reliable method of extracting DNA and RNA from sweetpotato, Ipomoea batatas (L). Lam. Biotechnol. Lett. 27: 1841|1845. ¨PubMed

12. Kim, SH., Hamada, T., Otani, M., Shimada, T. Cloning and characterizati on of sweetpotato isoamylase gene (IbIsaI) isolated from tuberous root. Breeding Sci. 55: 453|458.

11. Kim, SH., Hamada, T., Otani, M., Shimada, T. Cloning and characterizati on of sweetpotato MADS-box gene (IbAGL17) isolated from tuberous root. Plant Biotechnol. 22: 217|220.

10. Kim, SH., Hamada, T., Otani, M., Shimada, T. Use of single-leaf cutting in the study of the expression of starch synthesis and modification genes in sweetpotato. J. Plant Biotech. 7: 123|127.

2004”N
9. Tomita, R., Hamada, T., Horiguchic, G., Iba, K., Kodama, H. Transgene overexpression with cognate small interfering RNA in tobacco. FEBS Lett. 573: 117|120. ¨PubMed

2002”N
8. Kuwabara, C., Takezawa, D., Shimada, T., Hamada, T., Fujikawa, S., Arakawa, K. A basic acid- and cold-induced thaumatin-like protein in winter wheat has an antifungal activity against snow mould, Microdochium nivale. Physiol. Plant. 115: 101|110. ¨PubMed

2001”N
7. Wakita, Y., Otani, M., Hamada, T., Mori, M., Iba, K. Shimada, T. A tobacco microsomal ω-3 fatty acid desaturase gene increases the linolenic acid content in transgenic sweet potato (Ipomea batatas). Plant Cell Rep. 20: 244|249.

1998”N
6. Hamada, T., Kodama, H., Nishimura, M. Takeshita, K., Utsumi, H., Iba, K. Characterization of transgenic tobacco with an increased α-linolenicacid level. Plant Physiol. 118: 591|598. ¨PubMed

1997”N
5. Nishiuchi, T., Hamada, T., Kodama, H., Nishimura, M., Iba, K. Wounding changes the spatial expression pattern of the Arabidopsis plastid ω-3 fatty acid desaturase gene (FAD7) through different signal transduction pathways. Plant Cell. 9: 1701|1712. ¨PubMed

1996”N
4. Hamada, T., Nishiuchi, T., Kodama, H., Nishimura, M., Iba, K. cDNA Cloning of a wounding-inducible gene encoding a plastid ω-3 fatty acid desaturase from tobacco. Plant Cell Physiol. 37: 606|611. ¨PubMed

3. Hamada, T., Kodama, H., Nishimura, M., Iba, K. Modification of fatty acid composition by over- and antisense-expression of a microsomal ω-3 fatty acid desaturase gene in transgenic tobacco. Transgenic Res. 5: 115|121. ¨PubMed

1994”N
2. Hamada, T., Kodama,H., Nishimura, M., Iba, K. Cloning of a cDNA encoding tobacco ω-3 fatty acid desaturase. Gene. 147: 293|294. ¨PubMed

1. Kodama, H., Hamada, T., Horiguchi, G., Nishimura, M., Iba, K. Genetic enhancement of cold tolerance by expression of a gene for chloroplast ω-3 fatty acid desaturase in transgenic tobacco. Plant Physiol. 105: 601|605. ¨PubMed

’˜‘

Hamada, T., Kodama, H. 2006. Phenotype of the Transgene in Plants: Expression and Silencing. Floriculture, Ornamental and Plant Biotechnology Vol.II. pp. 98|107. Global Science Books.





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